Why has Richard Wagner been a controversial composer even today Essay Example

Why has Richard Wagner been a controversial composer even today Essay As in religious and political questions, when race has once been admitted, it becomes a factor of the highest importance in musical controversy. A prominent part in the music and literature of Germany in the 19th century played the popular, but controversial as well, German composer, conductor, theatre director and essayist Richard Wagner. His full name actually is Wilhelm Richard Wagner. Born in Leipzig, Germany on May 22, 1813, unlike most other great opera composers, Wagner wrote both the scenario and libretto for his works. He is primarily known for his operas, called later music dramas. He is one of the composers who revolutionized opera by writing stage works of unprecedented length and complexity , in which the orchestra was no longer a simple accompaniment to the singers, but an integral part of the action. Wagner made the music continuous, unlike the previous tendency to make an opera as a collection of separate arias and ensembles which could be performed separately . He used a complex system of what are called Leitmotifs, or melodic ideas which stand for characters , things or ideas in the drama. With Wagner, harmonies became more complex than ever before, and his revolutionary use of this element influenced composers everywhere, and led to the music of the 20th century. As I already mentioned, Wagner is the most controversial composer of 19th century. He divides people as no other composer did it before. A lot of people and musical critics find his work magnificent, but there are a lot of others who cant stand his music, because of his ideas, mostly political opinions in the real life. We will write a custom essay sample on Why has Richard Wagner been a controversial composer even today specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Why has Richard Wagner been a controversial composer even today specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Why has Richard Wagner been a controversial composer even today specifically for you FOR ONLY $16.38 $13.9/page Hire Writer Even a century ago, many critics and composers thought his music was awful noise, with the most cacophonous harmonies, but others recognized its greatness. Wagners operas, writings, his politics, beliefs and unorthodox lifestyle made him a controversial figure during his lifetime. The debate about his ideas and their interpretation, particularly in Germany during the 20th century, continued to make him politically and socially controversial in a way that other great composers are not. The biggest issue is generated by Wagners comments on Jews, which continue to influence the way that his works are regarded, and by the essays he wrote on the nature of race from 1850 onwards, and their putative influence on the anti-Semitism of Adolf Hitler. In some of his essays, Wagner attacks Jewish contemporaries, especially Felix Mendelssohn, and accused Jews of being a harmful and alien element in German culture. Wagner stated the German people were repelled by their alien appearance and behavior. He argued that because Jews had no connection to the German spirit, Jewish musicians were only capable of producing shallow and artificial music. They therefore composed music to achieve popularity and, thereby, financial success, as opposed to creating genuine works of art. Thats why, Wagner was the Hitlers favorite composer and he made his name a symbol of the Nazis. As a result of his infatuation, Hitler adopted many parts of Wagners thought and used this as propaganda that would influence World War II. As a result Wagners operas cannot be found in the modern state of Israel although his works have been broadcasted on government-owned TV and radio. To this day, those who attempt staging public performances are halted by protests, including protests from Holocaust survivors. As a great example, I have watched, the great from the filmmaking point of view, Triumph of the Will the most dramatic ever made propaganda film by Leni Riefenstahl, which chronicles the 1934 Nazi Party Congress in Nuremberg. The film opens with shots of the clouds above the city, and then moves through the clouds to float above the assembling masses below, with the intention of portraying beauty and majesty of the scene. The shadow of Hitlers plane is visible as it passes over the tiny figures marching below, accompanied by music from Richard Wagners Die Meistersinger von Ni rnberg, which slowly turns into the Horst-Wessel-Lied. Among Wagners ideas was that the opera be an uninterrupted melody, also emphasizing drama as a focal point. To Wagner, the opera was the ultimate blend of all the arts. Spending many years in exile, he went for long periods without hearing the performance of his own works. It is exceedingly difficult for us of the present generation to do justice to Wagner, or to arrive at any final and impartial conclusions concerning the permanent value of his gigantic achievement. As a conclusion, today Wagner is no longer a symbol of Nationalism and Nazism, as he was at the beginning of the controversy. Like Lili Eylon in her essay Wagner Controversy says: The question is not whether Wagners music is of high or low quality, nor is the argument about how deep-seated was his anti-Semitism really relevant. There is no doubt that there have been other composers who were no less anti-Semitic. While it cannot be maintained that Wagner was directly responsible for German National Socialism, there is no doubt that he was a powerful symbol in the Nazi era, and his music held a singular importance in the Nazi psyche. For those who survived the Holocaust, Wagners music is terrible reminder of the horror those people have gone through. For them music must be separated from the politics, but unfortunately Wagner was the composer, who actually merged them together and became the composer of music and race.

Learn About Photosynthesis Formula

Learn About Photosynthesis Formula Some organisms need to create the energy they need to survive. These organisms are capable of absorbing energy from sunlight and using it to produce sugar and other organic compounds such as lipids and proteins. The sugars are then used to provide energy for the organism. This process, called photosynthesis, is used by photosynthetic organisms including plants, algae, and cyanobacteria. Photosynthesis Equation In photosynthesis, solar energy is converted to chemical energy. The chemical energy is stored in the form of glucose (sugar). Carbon dioxide, water, and sunlight are used to produce glucose, oxygen, and water. The chemical equation for this process is: 6CO2 12H2O light → C6H12O6 6O2 6H2O Six molecules of carbon dioxide (6CO2) and twelve molecules of water (12H2O) are consumed in the process, while glucose (C6H12O6), six molecules of oxygen (6O2), and six molecules of water (6H2O) are produced. This equation may be simplified as: 6CO2 6H2O light → C6H12O6 6O2. Photosynthesis in Plants In plants, photosynthesis occurs mainly within the leaves. Since photosynthesis requires carbon dioxide, water, and sunlight, all of these substances must be obtained by or transported to the leaves. Carbon dioxide is obtained through tiny pores in plant leaves called stomata. Oxygen is also released through the stomata. Water is obtained by the plant through the roots and delivered to the leaves through vascular plant tissue systems. Sunlight is absorbed by chlorophyll, a green pigment located in plant cell structures called chloroplasts. Chloroplasts are the sites of photosynthesis. Chloroplasts contain several structures, each having specific functions: Outer and inner membranes- protective coverings that keep chloroplast structures enclosed.Stroma- dense fluid within the chloroplast. The site of conversion of carbon dioxide to sugar.Thylakoid- flattened sac-like membrane structures. The site of conversion of light energy to chemical energy.Grana- densely layered stacks of thylakoid sacs. Sites of conversion of light energy to chemical energy.Chlorophyll- a green pigment within the chloroplast. Absorbs light energy. Stages of Photosynthesis Photosynthesis occurs in two stages. These stages are called the light reactions and the dark reactions. The light reactions take place in the presence of light. The dark reactions do not require direct light, however dark reactions in most plants occur during the day. Light reactions occur mostly in the thylakoid stacks of the grana. Here, sunlight is converted to chemical energy in the form of ATP (free energy containing molecule) and NADPH (high energy electron carrying molecule). Chlorophyll absorbs light energy and starts a chain of steps that result in the production of ATP, NADPH, and oxygen (through the splitting of water). Oxygen is released through the stomata. Both ATP and NADPH are used in the dark reactions to produce sugar. Dark reactions occur in the stroma. Carbon dioxide is converted to sugar using ATP and NADPH. This process is known as carbon fixation or the Calvin cycle. The Calvin cycle has three main stages: carbon fixation, reduction, and regeneration. In carbon fixation, carbon dioxide is combined with a 5-carbon sugar [ribulose1,5-biphosphate (RuBP)] creating a 6-carbon sugar. In the reduction stage, ATP and NADPH produced in the light reaction stage are used to convert the 6-carbon sugar into two molecules of a 3-carbon carbohydrate, glyceraldehyde 3-phosphate. Glyceraldehyde 3-phosphate is used to make glucose and fructose. These two molecules (glucose and fructose) combine to make sucrose or sugar. In the regeneration stage, some molecules of glyceraldehyde 3-phosphate are combined with ATP and are converted back into the 5-carbon sugar RuBP. With the cycle complete, RuBP is available to be combined with carbon dioxide to begin the cycle over again. Photosynthesis Summary In summary, photosynthesis is a process in which light energy is converted to chemical energy and used to produce organic compounds. In plants, photosynthesis typically occurs within the chloroplasts located in plant leaves. Photosynthesis consists of two stages, the light reactions, and the dark reactions. The light reactions convert light into energy (ATP and NADHP) and the dark reactions use the energy and carbon dioxide to produce sugar. For a review of photosynthesis, take the Photosynthesis Quiz.

Strategy evaluation for barclays Essay Example | Topics and Well Written Essays - 1750 words

Strategy evaluation for barclays - Essay Example The present strategy of Barclays bank was effective because it led to a statutory increase of its income to  £11,461 in the year 2013 which is given in figure 3. The profit of the bank also increased to  £2868 in the same year compared to 2012 in which its gain was only  £ 797. The suitability of the strategy adopted by the bank by transforming its plan in order to convert it into ‘Go-To’ financial institution reaped huge benefits. The balance sheet of the company strengthened and its operations became efficient. Its core franchises like UK retail, Corporate and Investment banking witnessed sufficient growth. A look at the share price of Barclays bank in figure 4 highlights that the turnaround plan was successful in improving the performance of the company. However, the executive chairperson of the bank McFarlane opined that there are certain pitfalls in its strategy implementation that needs to be mitigated in order to accelerate its progress. The strategic positi on of Barclays bank illustrated in figure 5 reveals that in terms of leadership, it occupies the highest position but HSBC is far ahead in terms of status. Other banks like Standard Chartered and RBS have succeeded in engagement more than Barclays bank. However, Barclays need to move its position in the engagement quadrant in order to compete with its rivals. Out of all the alternatives, the bank has selected the second one by serving its products in different countries in order to create its presence internationally.

How Safe IS The Biodiesel for Air Transportation Essay

How Safe IS The Biodiesel for Air Transportation - Essay Example This paper will evaluate the safety of using biodiesel in air transportation. Significance of Air Transportation Critics have challenged the probability of Air transport being the best mode of transport despite the many advantages attributed to it. Irrespective of this, this form of transport is of great significance because it has offered the globe a fast and reliable connectivity. The industry has introduced realms of possibilities. Traversing continents takes a few days or hours depending on the choice of flight connectivity. Air transport is very crucial for global trade especially trade surrounding perishable goods. Tourism is an additional sector that is only a possibility because of air transport. Tourism is an economic stronghold in many nations and contributes immensely to economic growth. Air transportation makes it is simpler for people to import and export culture. In the past, this critical sector has relied on the use of petroleum diesel for its engines. Despite the num erous technological changes that surround the aviation industry, the industry has persisted in its dependence on fossil fuels. However, the recent environmental concerns about the emission of greenhouse gases by fossil fuels, and the resulting consequences have compelled experts to embark on the search for reliable and cleaner sources that promise both economical and environmental sustainability (Pandey 34). Fossil fuels have threatened the environment with the uncontrollable emission of greenhouse gases and hydrocarbons into the environment initiating and enhancing global warming. The search for alternative energy sources has identified biodiesel as a reliable energy source. More importantly, future prospects indicate that biodiesel will be a reliable source to drive the aviation industry. Analysis of the potential benefits of biodiesel presents a promising future. Compared to fossil fuels, biodiesel releases zero percent of sulfates while fossil fuels release a high percentage of these (Metz 30). Moreover, with biodiesel, the aviation industry has the assurance of 78 percent less carbon dioxide in the atmosphere. Such immense reduction of carbon dioxide has been the central focus of many researchers in the recent past. Intensive research has revealed the different cycles of fossil use and biodiesel use. In the fossil use case, each step involves the release of carbon monoxide into the atmosphere (Pandey 56). However, the cycle does exhibit any possibility of recycling in the atmosphere. On the contrary, fossil fuels bring a new supply of carbon dioxide into the atmosphere from the ground. On the other hand, biodiesel use exhibits a possibility of recycling. Sources of biodiesel include feedstock from oils, plants, and algae. Plants utilize carbon dioxide that is available in the atmosphere for the manufacture of carbohydrates that are very crucial for metabolism. Extraction of biodiesel from plant feedstock can produce either first generation of second gener ation biodiesel. Although the processing and use of the biodiesel in aviation releases carbon dioxide to the atmosphere, it does not destruct the carbon dioxide cycle because the amount released becomes critical for the growth of more plants (Ellman 120). Replacing petroleum diesel used in the aviation industry with biodiesel will serve to eliminate the additional release of carbon dioxide to the atmosphere from fossil fuels. This will reduce the 78 percent of carbon dioxide

NATURAL HAZARDS VOLCANOES Essay Example | Topics and Well Written Essays - 500 words

NATURAL HAZARDS VOLCANOES - Essay Example (Adams, 1981). 2. Volcanoes are very likely to erupt on these islands due to the existence of ‘live’ volcanic centers. It is more likely to occur in other countries such as Nevis, Eustatius and Saba in the near future because they have live volcanic centers. (University of the West Indies Seismic Research Centre) 4. When a super-volcanic eruption occurs, usually it covers extensive areas with volcanic ash and lava that results in a prolong weather change such as the reduction in the temperature of the atmosphere and the earth’s surface, referred to as ice age or glacial age, which can threaten the life of species. (Wikipedia, the free encyclopedia). Volcanic eruptions, though disruptive and destructive to human, the environment and animal life, provides immense benefits to people who live near to them. The ash that is thrown when there is a volcanic eruption can be very harmful to the environment shortly after, however in the long term the ash layer converts to a very fertile soil, which contains many useful minerals. Volcanoes can produce very magnificent scenery like the beautiful sunsets from explosive eruptions.   They also create beautiful lava fountains, stunning eruptions and plant-rich environments. People will return and live after a volcanic eruption because of the fertile soil around the volcano, where they use the rich soil for farming.  Ã‚  Ã‚  Ã‚   The resources that are produced from volcanic eruptions are used for energy extraction, also called geothermal resources.   Energy is produced as a result of heat from the earths crust.   The great advantages are that this energy is very clean and the resources are almost inexhaustible. Another great economical benefit of volcanic eruptions is that it generates tourism. This translates to jobs and foreign exchange income. This can also result in an improvement in people’s standard of living.

Quantitative Real Time Polymerase Chain Reaction (RT-qPCR)

Quantitative Real Time Polymerase Chain Reaction (RT-qPCR) QUANTITATIVE REAL TIME POLYMERASE CHAIN REACTION (RT-qPCR) Primers All primer sequences were designed using the online tool Primer 3-BLAST (NCBI) and the primers were obtained from Sigma Aldrich, Bangalore, India. Relative expression of transforming growth factor beta (TGF- ÃŽ ²), myosin heavy chain beta (ÃŽ ²-MHC), endothelial nitric oxide synthase (eNOS) and glyceraldehydes-3-phosphate dehydrogenase (GAPDH) was studied. Forward and reverse primers for the above genes were used for amplification. Table 5. PCR Primer details RNA isolation All glasswares were rinsed with diethyl-pyrocarbonate (DEPC) treated water to inhibit RNases. Total RNA was isolated using guanidium thiocynate-chloroform-phenol method of Chomczynski and Sacchi (1987). Total RNA isolation kit (BioUltra, Sigma Aldrich,USA) was utilized for this study After cleaning with saline, heart and aorta tissues were homogenized in denaturing solution with freshly added ÃŽ ²-mercaptoethanol. After homogenization 2M sodium acetate solution (pH. 4.0), water saturated phenol and chloroform: isoamyl alcohol (49:1) was added. The mixture was shaked vigorously and allowed to cool on ice for 15 minutes. The mixture was centrifuged at 10,000 Ãâ€" g for 20 minutes at 4 oC. The aqueous phase was transferred in a fresh tube and an equal volume of ice cold isopropanol was added. RNA was precipitated by placing the sample at -20 oC for one hour. Then the mixture was centrifuged at 10,000 Ãâ€" g for 20 minutes at 4 oC. The pellet was washed with 70% ethanol and RNA was stored in DEPC water at -80 oC. RNA quality and quantity was assessed by nano-drop spectrometer. Real time PCR amplification SYBR Green Quantitative RT-qPCR Kit was used in this study and the PCR experiment was carried out in eppendorff realplex mastercycler. 1 µg RNA was reverse transcribed by using Molone murine leukemia virus (M-MuLV) reverse transcriptase as per manufactures instructions. Then the amplification program (94 oC – 45 seconds, annealing – 45 seconds, extension 72 oC- 1 minute) was applied with specific annealing temperature. The annealing temperatures of TGF-ÃŽ ², ÃŽ ²-MHC, eNOS and GAPDH were 58, 52, 55, and 55 oC, respectively. The specificity of the primers was confirmed by resolving the PCR products in 1.5% agarose gel electrophoresis. The relative fold change of expression was calculated by normalized the expression with GAPDH. The RT-qPCR results were quantified using the ‘threshold line’ and the ‘cycle threshold’. The ‘threshold line’ is the point at which the reaction reaches a fluorescent intensity above background. The cycles at which the samples reach this level is called the ‘cycle threshold’ (Ct). The statistical analysis of the RT-qPCR results was calculated by using the à ¢Ã‹â€ Ã¢â‚¬  Ct = (Ct value of gene of interest – Ct value of GAPDH). Relative gene expression was obtained by à ¢Ã‹â€ Ã¢â‚¬  Ãƒ ¢Ã‹â€ Ã¢â‚¬  Ct methods (à ¢Ã‹â€ Ã¢â‚¬  Ct sample – à ¢Ã‹â€ Ã¢â‚¬  Ct of control), with the use of the control group as a calibrator for comparison of all unknown sample gene expression levels. The relative gene expression fold change was derived from 2–à ¢Ã‹â€ Ã¢â‚¬  Ãƒ ¢Ã‹â€ Ã¢â‚¬  Ct (Schmittgen and Livak, 2008). IMMUNOHISTOCHEMICAL LOCALIZATION (IHC) Immunohistochemistry (IHC) IHC was performed as described by Rocha et al., (2009) using Super Sensitive Polymer-HRP Detection System kit, from Biogenex, USA. The Super Sensitive Polymer-HRP Detection System is a atypical detection system using a non-biotin polymeric technology that makes use of two major components: a Poly-HRP reagent and super Enhancerâ„ ¢. As the system is not based on the biotin-avidin system, the problems associated with endogenous biotin are completely eliminated. The detection of antigens in tissues by immunostaining is a two-step process. The first step involves the binding of an antibody to the antigen of interest and the second step involves the detection and visualization of bound antibody by one of a variety of enzyme chromogenic systems. The choice of detection system will dramatically impact the sensitivity, utility and ease-of-use of the method. Procedure Paraffin-embedded tissue was cut to obtain sections of about 4  µm thickness. The mounted paraffin-embedded slices are deparaffinized in xylene and rehydrated using an ethanol/H2O gradient. Heat mediated antigen retrieval step was carried out for 10 min and then the slides were allowed to cool to room temperature for another 20 min. This was followed by peroxidase block treatment (to block endogenous peroxidase enzyme activity) for 10-15 min and then power block treatment (to block non-specific binding of antibodies to highly charged sites) for another 15 min. The sections were incubated with the concerned diluted primary antibody solution (for 2 h (1:200)) followed by treatment with the super enhancer solution (for 30 min) and super sensitive Poly-HRP solution (for 30 mins). After colour development with DAB and counterstaining with haematoxylin, the sections were observed under the microscope and photographs were taken. TRANSMISSION ELECTRON MICROSCOPIC STUDY The ultrastructure of the heart specimen was examined by Transmission Electron Microscopy (TEM) according to the method of Lang (1987), by the technique of thin sectioning. Reagents Glutaraldehyde solution: 3% Osmium tetroxide: 2% osmium tetroxide in 10 mM sodium phosphate buffer, pH -7.4 Ethanol: 75%, 95% and 100% Uranyl acetate: 1% Lead citrate: 3% Sodium phosphate buffer: 0.1 M, pH 7.4 Procedure Immediately after the sacrifice, the heart tissues were dissected and fixed with a solution of 3% glutaraldehyde for 2 hours at room temperature and washed thrice with phosphate buffer to remove glutaraldehyde. Post-fixation was done by a solution containing 2% osmium tetroxide in 10mM sodium phosphate buffer and left overnight. Then, the osmium tetroxide solution was removed and replaced with 75% ethanol. This reduces the remaining osmium tetroxide to osmium dioxide, which forms a precipitate in the alcohol. After 10 minutes, the alcohol was replaced with a few ml of 75% ethanol. After 30 minutes, the alcohol was replaced with 95% ethanol and left for 30 minutes. This solution was replaced with 100% ethanol and washed thrice and then dried in acetone. After dehydration, the tissues were equilibrated for 30 minutes in 1:1 mixture of epoxy propane and the embedding medium, epon 812 (also called epikote resin-812). A mixture of the resin and two hardening agents, dodecyl succinic anhydride and methyl anhydride were used. A diamine catalyst generally N-benzyl-N-diethylamine was added just before use. The 1:1 mixture was poured off and replaced with full strength resin. This step was repeated several times to ensure full infiltration of the embedding medium. The tissue was then transferred to a beam capsule with a wooden stick and the capsule was filled with fresh resin mixture. The wooden stick was used to tease the specimen down to the center of the bottom of the capsule. Next, the block holder was placed with the specimen in hot air oven at 60 °C for 48 hours to polymerize the resin completely. Once the blocks are hardened, they are ready for sectioning. The ends of the specimen blocks were trimmed using glass knives and ultra thi n sections were cut using an LKBUM4 ultramicrotome. The sections were picked upon carbon grids and post-stained with combined uranyl and lead stain and rinsed with distilled water and dried. After drying, the grids were examined under a Philips EM201C transmission electron microscope (Philips, Eindhoven, Netherlands). WESTERN BLOT ANALYSIS Western blotting was performed to analyze the expression pattern of eNOS in the aorta and reperfused hearts according to method of Laemmli (1970). Principle Following the protein estimation, the samples were separated using SDS-PAGE gel electrophoresis and the separated molecules are blotted onto a polyvinylidene fluoride (PVDF) membrane. After blocking, the primary antibody was added and allowed to bind to the protein followed by washing (which removes non specifically bound antibody); then an enzyme-labeled secondary antibody was added, to detect the primary antibody. The location of the secondary antibody was determined by adding an appropriate substrate for the enzyme conjugated to the secondary antibody. Reagents Acrylamide stock: 30% acrylamide, 0.8% N,N†²-methylene bisacrylamide Separating gel buffer: 1.5 M Tris, pH 8.8 Sample buffer: 0.5 M Tris, pH 6.8 Sodium dodecylsulfate (SDS): 10% Ammonium per sulfate (APS): (10%) N,N,N,N-tetramethylethylenediamine (TEMED) Separating gel overlaying solution: Water-saturated isobutanol Sample Buffer: Tris (0.5M, pH 6.8)-2.5 mL SDS (10%)-4.0 mL Glycerol (100%)-2.0 mL ÃŽ ²-Mercaptoethanol-0.8 mL (or 1 M DDT-0.5 mL) Bromophenol Blue (0.1%)-300  µL Distilled water (400  µl) to 10.0 mL Running gel buffer Tris-6.05 g Glycine: 28.80 g 10% SDS: 10.0 mL or (1.0 g) Distilled water to 1000 mL Staining solution Coomassie brilliant blue R250- 300 g Methanol-80 mL Acetic acid-20 mL Distilled water-100 mL Destainning solution Acetic acid-100 mL Methanol-300 mL Distilled water: 1000 mL Procedure The aortic tissues were homogenized in an ice-cold radio immuno precipitation buffer (RIPA) (1% Triton, 0.1% SDS, 0.5% deoxycholate, 1 mM/L EDTA, 20 mM/L Tris (pH 7.4), 150 mM/L NaCl, 10 mM/L NaF, and 0.1 mM/L phenylmethylsulfonyl fluoride (PMSF)). The homogenate was centrifuged at 10,000 Ãâ€" g for 20 min at 4 °C to remove debris and the supernatant was used to determine the protein concentration of the lysates using the BCA protein assay kit (Merck, India). Transfer of proteins to membrane Samples containing 50 ÃŽ ¼g of total cellular proteins were loaded and separated using 10% SDS polyacrylamide gel electrophoresis. Following electrophoresis, the proteins were transferred from the gel to a membrane by using semi-dry blotting system (AA Hoefer, SEMIDRY BLOTER, USA). Before assembling the transfer system, soaked PVDF membrane in methanol for 10 minutes and blotting papers in cold transfer buffer. Prepared sandwich, blotting paper, membrane, gel and blotting paper, were placed in the transfer apparatus and few drops of transfer buffer was added and subjected to an electric current 20 V for 1 h under cold condition. After the transfer, the sandwich was removed from the transfer system. Membrane was stained with 0.5% ponceau in 1% acetic acid to confirm equal loading and then washed with distilled water. The PVDF membrane were blocked with 5% blocking solution (containing 5% BSA in 0.5 M Tris-buffered saline, pH 7.5) for 2 h to reduce the non-specific protein binding sites and then incubated with primary antibody (anti-eNOS), in blocking solution with gentle shaking overnight at 4 °C. After this, the membranes were washed with TBST (Tris-buffered saline and 0.05% Tween-20 (TBST)) thrice for 10 minutes interval and then incubated with respective secondary antibody anti-mouse IgG (Sigma-Aldrich, USA) conjugated to horseradish peroxidase. Then the membranes were washed with TBST thrice for 10 minutes interval. The reaction was developed with a DAB detection system (Merck, India). Bands were scanned using a scanner and quantitated by Image J, a public domain Java image processing software, Wayne Rasband, NIH, Bethesda, MD, USA. H9c2 cardiomyoblast cell culture Rat embryonic cardiomyoblast derived H9c2 cells was obtained from National Centre for Cell Science (NCCS), Pune, India. Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum and a combination of penicillin-streptomycin (1%) in a humidified 5% CO2atmosphere at 37 °C. The assay was performed by seeding H9c2 cells in the concentration of 1Ãâ€"104 cells/well in 96-well plate. In vitro oxidative stress and mitochondrial transmembrane potential study In order to evaluate the cytotoxic effect, viability was checked with MTT assay on D-carvone (25–100  µM) treated H9c2 cells. For assessment of protective potential of D-carvone against oxidative stress, different concentrations of D-carvone (0.1, 1 and 10  µM) were incubated with H9c2 cells for 2 h, and then co-incubated with 500  µM/L H2O2 for further 18 h (Jia et al., 2012; Zhang et al., 2011). For viability analysis, MTT solution (5 mg/mL) was added to each well, and incubated for 4 h at 37 °C. After incubation, optical density (OD) was measured on a microplate reader at 570nm. With the 10  µM dose of D-carvone, the level of intracellular ROS formation was quantified with fluorimetry using redox-sensitive fluorescent probe 2, 7-dichlorodihydrofluorescin diacetate (DCFH-DA). Further, to examine mitochondrial membrane permeability transition (MPT), H9c2 cells were incubated with 5 mg/mL Rhodamine 123 (Rh123) at 37 °C for 30 minutes (Park et al., 2003). The images were acquired using the Olympus IX71 inverted à ¯Ã‚ ¬Ã¢â‚¬Å¡uorescence microscope. Ischemia/reperfusion (I/R) protocol D-carvone was dissolved in 1% DMSO (vehicle) and administered orally to rats using an intragastric tube daily for 7 days. The rats were randomly divided into four groups of six rats per group: (i) control group pre-treated with vehicle alone for 7 days (isolated rat hearts subjected to continuous perfusion). Isolated rat hearts obtained from the following three groups were perfused with a modified Krebs buffer solution for 10 minutes to stabilize the cardiac functions and then subjected to 30 minutes of global ischemia, followed by 60 min of reperfusion: (ii) I/R hearts pre-treated with vehicle alone for 7 days (Control (I/R)); (iii) I/R hearts pre-treated with D-carvone (I/R + D-C 10 mg/kg body weight); (iv) I/R hearts pre-treated with D-carvone (I/R + D-C 20 mg/kg body weight). Langendorff isolated heart preparation The animals were anaesthetized with an intramuscular injection of ketamine (75 mg/kg body weight). After thoractomy, the hearts were rapidly excised and placed in cooled (4 °C) Krebs Henseleit bicarbonate solution [composition (in mM): 118 sodium chloride (NaCl), 4.7 potassium chloride (KCl), 1.2 magnesium sulphate (MgSO4), 1.2 potassium dihydrogen orthophosphate (KH2PO4), 2.3 calcium chloride (CaCl2), 25.0 sodium bicarbonate (NaHCO3), 11.0 glucose]. composition (in mM): 118 sodium chloride (NaCl), 4.7 potassium chloride (KCl), 1.2 magnesium sulphate (MgSO4), 1.2 potassium dihydrogen orthophosphate (KH2PO4), 2.3 calcium chloride (CaCl2), 25.0 sodium bicarbonate (NaHCO3), 11.0 glucose. The heart was then attached to the cannula through aorta and retrogradely perfused with the Krebs solution maintained at 37 °C and continuously gassed with a mixture of 95% O2 5% CO2. Perfusion pressure was kept constant at 80 mmHg. The ischemia and reperfusion protocol was followed as described previously (Khan et al., 2006; Senthamizhselvan et al., 2014). An elastic water-filled balloon was introduced into the left ventricle through a left atrial incision and connected to a Pressure Transducer (AD Instruments) linked with a PowerLab data acquisition unit (AD Instruments). The balloon volume was adjusted to achieve a stable left ventricular end-diastolic pressure (LVEDP) of 5-10 mmHg. The percentage rate-pressure product [RPP = (LVSP-LVEDP) Ãâ€"HR] and percentage coronary flow was assessed as described previously (Esterhuyse et al., 2005; Ferrera et al., 2009; Swaminathan et al., 2010). Coronary effluent was collected for the estimation of LDH activity. Macroscopic enzyme mapping of infarcted myocardium (Triphenyl Tetrazolium Chloride test) TTC (triphenyl tetrazolium chloride test) test used for a section of the heart tissue. Lie et al. (1975) method was used for the triphenyl tetrazolium chloride test (TTC) analysis acclimated for the macroscopic enzyme mapping appraisal of the infarcted myocardium was completed. A freshly prepared solution of 1% TTC in phosphate buffer was prewarmed at 37-40 °C for 30 minutes in a darkened glass. To remove the excess blood, the heart tissues were washed rapidly in cold water without macerating the tissue. After removing epicardial fat, the left ventricle was taken separately. To obtain slices not more than 0.1-0.2 mm in thickness, the heart was transversely cut across the left ventricles. The heart tissue slices were kept in the covered, darkened glass dish containing prewarmed solution of TTC and the dish was kept in an incubator and heated to 37-40 °C for 45 minutes. The heart slices were turned over thrice and made certain that it remains fully immersed in the TTC solution. At the end of the incubation period, kept the heart slice in fixing solution to fix the tissue. Colour photographs of slices were obtained by a camera with macro lens. The expected reaction of the TTC test was as follows: normal myocardium (LDH enzyme active) turned to bright red, infarcted myocardium (LDH enzyme deficient) turned to uncolored white.

Business Plan - Nursery ::

I have selected a nursery because there is high demand in the UK as well that there is a reasonable amount of profit SUMMARY PEOPLE I intend to employ on 3 to 4 employees along with my self and cater for approximately 12-20 kids once the business has established itself then I may decide to expand and cater for more children. THE PRODUCT I have selected a nursery because there is high demand in the UK as well that there is a reasonable amount of profit, which can be made if the business is planned out correctly. One of the main reasons why demand is so high is that more women then ever before are going to work, and therefore day nurseries provide a suitable environment for their children to be looked after in. THE MARKET ---------- One of the main reasons why demand is so high is that more women then ever before are going to work, and therefore day nurseries provide a suitable environment for their children to be looked after in. PRODUCTION Since my business is basically a service that is a day nursery hence there is no production involved it is the services, which I provide to my customers. I offer milk to the kids, if any of the kid needs milk I will be offering them, then there will be a rest room in which those kids who are tired can sleep in that room, the third service which I offer is playing, I will be having a room in which all the toys will be there all the kids can play there, my childminders will play with them, and the fourth service is giving the kids basic education. EXPECTED PROFIT For my business to be successful it is very important that it should make profit , so at the end of one year I will be making a profit of  £2454 which shows that my business is going to do well, its just the starting as and when my nursery becomes popular among the people I thing it will be doing well and making more profit. FINANCE NEEDED As mentioned in financial and production plan I will be looking to obtain a loan of  £5000 from the bank. If the bank rejects my application, then I will consider getting a sleeping partner so that I can apply again for lower amount. I could also find a partner and then half the investment. This is because currently I have personal savings of  £3500 and could use  £2500 of this to open my business with my partner. When discussing the plan with my father, he agreed to help

Human Resource Essay

Question 1: How would you forecast the manpower needs of Ya Kun? Ya Kun can forecast their manpower by using trend analysis, ratio analysis, scatter plot and computerized system. The personnel can use either one of these method of finding new recruitment. Based on trend analysis, Ya Kun find new recruit by using their past experienced which means that they can provide an initial estimate of future staffing needs, but employment levels rarely depend just on the passage of time. They looked their past performance of sales, productivity and so on to estimate their employee also because if their performance goes beyond their expectation like Ya kun that have many outlets throughout the world, they may required more employee either full time or part-time. Other forecasting method that Ya Kun can use to forecast their manpower is ratio analysis. In this method, Ya Kun forecast based on the historical ratio between some causal factor (like sales volume) and the number of employee required such salespeople. In this case, Ya Kun need more employee because they need more salespeople to run the business in each of their outlets. The sales volume for Ya Kun also one of the factor that Ya Kun need to recruit employee. Compared to trend analysis, ratio analysis assumes that productivity remains about the same. Personnel also can use scatter plot to forecast their manpower. In this method, the personnel may use two related variables to indicate the relation between the two such as sales volume and number of employee. If these two factors are related, then the points will tend to fall along straight line then the personnel can forecast the need of new recruitment. Computerized forecasting also is one way that personnel can use to forecast their need of manpower. With programs like these, employers can more accurately estimate how many employee that they need to projected productivity and sales. Many firms use computerized employee forecasting system for particularly for estimating short-term needs. In Ya Kun case, their personnel may need this program because they have many outlets that need more part-time worker to replace fulltime worker if needed. Question 2: What are the advantages and disadvantages of Ya Kun’s hiring part-time workers? Part-time workers are those who whether by personal choice or due to employment conditions beyond their control work fewer hours than the regular, full-time staff of a business over the course of a year. There is no specific number of hours that makes someone full or part-time, but a full-time worker will usually work 35 hours or more a week. The advantages of Ya Kun’s hiring part-time workers are part-time workers are less expensive than full-time workers. This is because some of them are paid less than half of what full-time workers, who do the same work, are paid. In other hand, part-time workers will not get benefits same like full-time workers such as insurance or sick pay. So Ya Kun’s can save significant amount of money by hiring part-time workers. Most of the part-timers are student, since it allows them to balance their academic needs with their school needs and they are often willing to learn new tasks and responsibilities when doing their work. Therefore, they will do their job better than full-time workers and this will increase labour productivity of Ya Kun’s. Furthermore, Ya Kun’s only hiring part-time workers when the full-time workers go on vacation or become ill. Students are particularly useful in meeting these periods of high demand in order to ensure that the needs of Ya Kun ’s customers are met and will increase in Ya Kun’s output as well. The disadvantages of Ya Kun’s hiring part-time workers are Ya Kun’s need extra time to train part-time workers to get knowledge about their work and they also need to be guide by someone before they can do their work by their own. Besides that, it will increase training cost of Ya Kun’s. Part-time workers have to go for training before they can start their work in order to avoid from low skilled workers. This also will affect productivity of Ya Kun’s. Moreover, part-time workers have higher absenteeism rates. This is because most of the part-time workers are student and they only doing part-time work to get extra money or just for fun. Besides that, they do not think about their commitment so they keep absent to work. Question 3: A good attitude and commitment are two important attributes that Ya Kun looks for in its job applicant. Is a job interview an effective method to assess these two attributes? What else can Ya Kun do to get reliable information on these two attributes? It is good to have job interview, but conducting a case test and attitude test would help to assess the attribute. Recruiting via the internet: The recruiter should be given complete description about the job and who will be qualified to apply for the job. A simple test could be done to screen the applicants online. Afterwards, a job interview will be needed. Advertising: The employer should advertise their ads where they’ll reach your prospective employees. The recruiter should be given complete description about the job and who will be qualified to apply for the job. Afterwards, a job interview will be needed. Private employment agency: The recruiter should be given complete description about the job and who will be qualified to apply for the job. Afterwards, a job interview will be needed. Walk-ins: This involves direct interview, which is the effective way to measure good attitude and commitment rather than just simply looking at the resume. Question 4: What suggestion would you make to Ya Kun to improve its recruiting processes? From the case Ya Kun Kaya Toasts, we can see that Ya Kun used the recruitment and selection process through advertised in Chinese and English newspapers as well as trough recruitment notice at their outlets. There are few steps to applicants should be undergo before they can be select and recruit by Ya Kun Kaya Toasts. It start from the interview by two manager, screened primarily for their level of commitment and willingness to work shifts, screened for other desirable qualities, until when they successful then they need to go through 2 week training and remain on probation for 3 months. However, Ya Kun still thinking the way how to improve its recruiting process. The reasons why is because the main challenge Ya Kun face in its recruitment efforts is finding employees with the right attitude, because the technical skill required are relatively easy to learn. Other than that, there are some applicants were unwilling to work shifts, making it difficult for Ya Kun to hire them because shift work is inevitable in the food and beverage retail industry. Look at the case, challenge economic times are prompting employers especially Ya Kun manager to rethink how they go about recruiting process. So, there are several suggestions would we make thinks to Ya Kun can do to improve its recruiting processes. Firstly, from job opening for the outlet staff the point in your advertisement should reach your target prospective employees. Use the guide AIDA (attention, interest, desire, and action) to construct advertisements. You must attract attention to the advertisement. From that case, Ya Kun should state in the ad that all the responsibilities and qualifications required for work at Ya Kun. For example, under the responsibilities and qualifications required the applicants should be education because we know that the person who educated has the right attitude toward the job. Moreover, don’t forget to include the required for â€Å"work shifts interested only† in the advertisement. So that, during the interview period manager not wastes their time recruit the applicants. Secondly, recruitment materials have a more positive impact if they contain more specific information. That means, at the application form the manager should provide applicants with information on aspects of the job that are important to them, such as salary, location, and diversity. For example, pay the salary base on their schedule of shift work. As we look at the case, some applicants were unwilling to work shifts because they not satisfy about the salary especially when they work at second shift period from 3 P.M to 11 P.M. The reason because at that time, number of customer may be increase because that time are pass the work time and they may like to hang up at Ya Kun coffee shop with their friend and family. Moreover, the manager of Ya Kun also can do the job rotation which is the periodic shifting of a worker from one task to another. That means, results depend on how job satisfaction is measured. Thirdly, using application forms to predict job performance. In this stage, manager should already get all the application forms from the applicants who apply for that job after the certain period of job opening for the outlet staff or recruitment notice at their outlets. It is possible for the Ya Kun manager to use application form information to predict which candidates will be successful and which won’t, in much the same way that one might use test for screening. The basic process involves conducting statistical studies to analyze the relationship between biodata responses on the application form and measure of success on the job. It is important to choose the biodata items in order to look at their life style as well as other criteria such as their background, education, and others. So, we can evaluate the candidates during the interview session whether they pass or not. Conclusion It is important to understand the nature of the business that we are in before doing the recruitment and selection process. Different nature will result different way or step to look at the employees which is really suitable to apply as well as to be accept to work at that company or industry. From the case it start with personnel planning and forecasting where it is the process of deciding what position the Ya Kun industry will have to fill, and how to fill them. Filling open position with their internal sources of candidate make Ya Kun has several advantages. For example, Ya Kun has a family –style environment and established â€Å"promotion-from-within† policy and many more. Then as result, not only the organization build the good structure and enhance performance, but the emphasis on good attitude and character in the selection of outlet staff has help Ya Kun build a pool of hardworking and committed workers. Ultimately, the main goal that the Ya Kun will achieve is make it big through the improvement its recruiting process in the future. That all the improvement for Ya Kun has to face in order to take the challenge by increasing the commitment of all staff toward the company.

English Essay, intertextuality

English Essay, intertextuality Childhoods End and Columbine Whose Fault Is ItMy reading of "Childhoods End" by Lance Morrow did not influence my understanding of the issues raised in "Columbine: Whose Fault is it?" by Marilyn Manson. It did not influence my understanding of the issues in Manson's article as Lance Morrow's article did not persuade me to think any certain way about the issues presented. Lance Morrow did not "bias" or "influence" my position on violence or guns, and hence didn't influence what I read in Manson's article.The article "Columbine: Whose fault is it" describes gun violence in today's society. Whilst Manson probably wrote it to stop people from blaming teenage violence on his music, there are also many other meanings and issues aside from this. It goes pretty deep into discussing violence in our world. It says that "we applaud the creation of a bomb whose sole purpose is to destroy all of mankind", and that "America puts killers on the cover of Time magazine.English: Marilyn Manson at the Eurockà ©ennes of 20...Although Mr Manson does state that times are not becoming more violent, just more televised, the fact is that media coverage has the potential to brainwash children into believing violence is "OK". It can sway them towards pulling the trigger on a gun, because they've seen it so many times on TV. This is what Manson tells us towards the end, "kids are growing up more cynical; they have a lot of information in front of them." "There's nowhere to run." So really, this shows that violence is on the rise, and it's the medias fault. They're practically 'teaching' us how to be violent. "The media, since their inception, have turned criminals into heroes".We try and find a reason to justify killing. That may be by blaming it on "evil" music...

Edouard Manet Essays - French Intervention In Mexico, Douard Manet

Edouard Manet Essays - French Intervention In Mexico, Douard Manet Edouard Manet Edouard Manet To artists, Edouard Manet was seen as a revolutionary who revealed his inner self to radically change the style of painting in the 1900s. Born on January 23, 1832, Manet was forced into studying law by his father, who was a high government official. As the eldest of 3 sons, the famous artist was expected to take up the tradition of work in the field of justice, which had been in the family for generations. However, Manet had a burning desire in his heart as a child, which told him law was not where he should be. When Manet was sixteen, his uncle, a passionate lover of art, noticed his nephews similar interest and got Manet into an art school in Paris called College Rollin. Unfortunately, Manets father was strongly against this idea because he wished Manet to continue studying law. After much feuding, the father and son came to a compromise that Manet would go into the Navy. Yet after only a year at sea Manet was completely miserable and reported back home to continue his education in art despite his fathers wishes. At the age of 18 he entered instruction under Thomas Couture. Manet continued under Coutures instruction but soon became bored with the conventionalism of his teachings and sought something more exciting. That led to travels to Italy where he began creating his masterpieces that changed the history of art. As the style of art began to radically change Manet became the founding father of the impressionistic era. He began taking everyday objects and pulling out of those objects new feelings and creations which provoke thought. With broad strokes Manet used a vivid summary technique. Manet adopted bold brush strokes, and emphasized certain characteristics considered unorthodox realism by the church. Pre-Manet paintings had flat and static qualities. He created a new type of painting that made people think, and made art reviewers and members of society angry and confused. 1 2 Manet painted what he saw as he saw it, not at all the common view. This concept forced onlookers to take new perspectives on things that had been so solid in their minds. Furthermore, Manet introduced astonishing brightness in his paintings and he used stark contrast. Fellow artists picked up on many of Manets styles and he in turn adopted the use of lighter colors and added more emphasis on the effects of light. Manets risk taking paintings created a new modern style for future artists to follow. Among the many Edouard Manet paintings are two rather interesting pieces. One of these is The Absinthe Drinker. Created in 1858-59, the 71- X 41 canvas painting features a drunken man leaning against a wall in a dark corner. As one of the first controversial paintings Manet created, it served as a lead-in into his career that was shunned by the public eye. The painting made no effort to sentimentalize the subject of drinking. The painting is enjoyable because of its simplicity and soft contrast. Manet blends the objects well to create a dark tone that also shades light in the right places. This work now stands in the NY Carlsberg Glypotetek in Copenhagen. Another painting that drew attention to Manets skill is Argenteuil. In this bright depiction of a day at the harbor, one might enjoy Manets bold bright contrast of colors. In his painting, which was created in 1874, Manet uses bold, sharp strokes to make the central objects project more. This piece, noticeably smaller then the previous one, being 57 7/8 X 44 , uses a magnificent blend of colors in oil on canvas. His use of new and different techniques and colors that stand out make this picture notable to the development of the artistic style of the impressionist period. This painting, a pillar of change, hangs in the Bayerische National Gallery in Munich. 3 Manets works inspired the impressionist style, influenced French panting and the general development of modern art. Hailed by young painters as their leader, Manet became the central figure in the dispute between the academic and rebellious art factions of his time. Furthermore, he influenced artists to try and open up new paths for themselves and for all painting. Not only

The cask of amontillado Essay Example | Topics and Well Written Essays - 250 words - 1

The cask of amontillado - Essay Example The narrator was cunning in his use of self-deprecation to keep his victim at ease. Montresor constantly employed stroking Fortunato’s ego and commending his fortune while belittling himself which the latter enjoyed and Montresor secretly disdained. â€Å"You are rich, respected, admired, beloved; you are happy, as once I was. You are a man to be missed. For me it is no matter† (Poe 11). Relentlessly, Montresor put on the show of bowing down to Fortunato and emphasizing their polar place in society. Both born of noble blood, the obvious loss of Montresor’s money and place in society became a source of his envy and spite. But though he may have been suffering from poverty, the inherent conceit of an aristocrat remains with him and throughout the story he hints of an insult that Fortunato committed against him. Though it was not spoken in specific terms exactly what humiliation Montresor had suffered, his lust for revenge was enough to fuel

General Biology - Summary and Review of Two Articles Article

General Biology - Summary and Review of Two Articles - Article Example Charles Darwin’s suggestion that life began from a warm little pond rich in nutrients also supported the concept; however, oceanographers still continue to find the oases of life on the seafloor. Meanwhile, a team of researchers discovered that the cellular fluid is very similar to condensed vapors found in volcanic mud pots on land and that terrestrial environments boast the high ratios of potassium and sodium compared to marine environments (Mosher, n.p.), which explains why cellular life probably began on land as cells are rich in potassium and sodium. The first cells were believed to have no complex proteins to pump excess sodium making them highly permeable and completely at the mercy of their environments. Geothermal fields on land such as mud pots are probably rich in potassium, an environment favorable to begin cellular life; however, scientists ignored the theory because the modern Earth is in acidic condition. I agree with what the scientists say that life began at primordial oceans. In my opinion, a warm, slimy mud fed by volcanically heated steam is not an ideal setting for cells to begin or either sustain life. I learned that a warm and moist environment could host bacterial cells but an environment with above boiling point temperature such as a volcanically heated steam could kill living cells thus, it is not possible for cellular life to begin in an environment that could kill them at once. In addition, I think that my perception about where cellular life began is true.